Soundscape ecology is the study of the acoustic relationships between living organisms,and their environment, wether in natural habitats or urban landscapes. One way to study soundscape is to use acoustic indicators which are mainly focused on biophonies. According to studies, no research has been conducted on the measurement of the acoustic indices and its relationship with biodiversity and landscape in Iran. In this study, 21 urban parks in Isfahan were selected for sampling of biophonies in spring which were sampled in each park by determining a specific station within 30 minutes. The biophonies of each park were recorded based on the singing of urban birds and their acoustic indicators were calculated by R software and compared with biodiversity indicators, noise pollution, landscape indicators and spectral vegetation density index and the significant relationship between them was statistically examined. In this study, the share of biophonies and human sounds in each park have been calculated. The share of Biophonies in each park has been different based on environmental variables and the acoustic indicators of these sounds have made a significant relationship with most biological variables. According to the results, Sofeh Park and north of Nazhvan Park have the highest share of biophonies and Mahmoudabad Park and Fadak Park has the lowest share of biophonies that these parks need more protection. Finally, urban parks were prioritized based on the number of indicators affecting the biophonies of each park. The results showed that Sofeh Park and North Nazhwan Park have the highest share of biophonie and Mahmoudabad and Fadak Parks have the lowest share of biophonies, consequently these parks need more restoration measures. In addition, there is a significant relationship between Bioacoustic Index (BI) and Normalized Difference Soundscape Index (NDSI), biodiversity indicators (Shannon and species richness), noise pollution (SPL and Leq) and landscape metrics (SIDI, SHDI, SIEI) that the correlation between these indicators is more than 0.6. SVM showed a very significant relationship between BI index and species richness (R2 = 0.82) and the number of singing birds (R2 = 0.87). According to the results of this study, the Bioacoustic Index (BI) and the Normalized Difference Soundscape Index (NDSI) can be a good way to predict biodiversity estimates, noise pollution and the complexity of urban landscape so that these indicators can also use as the main criterion for determining urban ecological parks. Acoustic indicators provide a better understanding of urban soundscape and urban physical space which can help in managing of urban green spaces. The complexity of the sound in urban parks can indicate the complexity of the landscape and the Variety of landuse and the development of urbanization Therefore, management considerations and restoration planning are required for soundscape conservation in urban parks of Isfahan. Keywords: Acoustic Indicators, Biophonies, Human Sounds, Soundscape, Urban Green Space