This project includs two sections: In first section، Six novel furandicarboxylate derivatives were proposed as fluorescence standards for instrumental calibration procedures and luminescence measurements. Their spectroscopical characteristics such as fluorescence quantum yields ( ? FL ) and chemiluminescence quantum yields ( ? CL ) were determined and compared to those of the universally accepted fluorescence standards. Their outstanding characteristics such as high fluorescence quantum yield، moderate chemiluminescence quantum yield، excellent stability، resistance to UV-induced photodegradation and simple and unstructured emission spectra make them preferable alternatives to the well-known and widely used fluorescence standards such as luminol and anthracene. In second section، A simple and fast procedure is described for evaluating the antioxidant activity of hydrophilic and hydrophobic compounds by using the chemiluminescence reaction of Bis(2،4،6-trichlorophenyl) oxalate (TCPO) with hydrogen peroxide in the presence of di(tert-butyl)2-(cyclohexylamino)-5-[(E)-2-phenyl-1-ethenyl]3،4-furandicarboxylate as a novel fluorophore. The AC 50 and AC 90 values of all studied antioxidant were calculated and the results were discussed on the basis of structure–antioxidant activity relationship. It was found that the proposed method is free of physical quenching and oxidant interference، for this reason، proposed method is able to determine the accurate scavenging activity of the antioxidants to the free radicals. Finally، the proposed method was applied to the evaluation of antioxidant activity of complex samples such as olive oils.