Recent development in technology and increasing lifetime, age related diseases such as musculoskeletal disorders have been increasing. One of the most important and efficient treatment methods of these disorders is total replacement of injured joint or so called arthroplasty. Accordingly, Ti6Al4V alloy could be efficiently utilized efficiently for knee and hip joints replacement. Main challenge in applying this alloy could be due to its weak tribological behavior and bio neutral. Therefore, the aim of present research is to improve biological and tribological properties of Ti6Al4V alloy through changes in surface topography of implant by laser and applying layered lubricant coating including graphene oxide- polyvinylpyrrolidones onto its surface.Therefore, at first, micro dimples with different surface density were deposited on the surface through laser, impact of scanning rate of laser rays (0.5-10 millimeter per second) on mechanical, chemical, biological and tribological properties was investigated, in following, optimal sample was selected. In this research, firstly, the effect of solvent (water: ethanol in various W/Vercent, water and Isopropyl alcohol), content of graphene oxide in suspension (0.5 and 1 gram/liter), voltage (10, 30, 60 and 90 V) and time (5 and 10 min) of electrophoretic on final coating properties were rated, followed by selecting optimal coating. In the second stage, polyvinylpyrrolidones and tanic acid were electrophoretically coated on alloy using a suspension containing various concentrations of polymer (0.3, 0.5 and 1 gr/li) and tanic acid (0.2, 0.3, 0.5 and 1 gr/li), constant deposition voltage and different times (3, 5 and 10 min) were considered in this stage. Consequently, optimal parameters of electrophoretic process were selected based on evaluation of coating morphology. Finally, the impact of formed micro tissues and two layer coating on tribological and biological properties was investigate. Based on the results, it was clear that the sample subjected to scanning rate of 5 mm/s with diameter and surface density of micropores respectively 190±5.5µm and 21.2±0.56 % was optimal sample in terms of hardness, biological and tribological properties. Maximum hardness of 635±21 Vickers, in constant distance of 1000 meters, in compared to standard sample (13.97±3.5), the mass loss reduction (13.1±2.5 mg) in wear test and the most area fraction by cell in cellular test. These rusules showd that, in sample with bes parameters in laser, the wear mechanism was changed from abeasive and plastic deformation and delamination to abrasive mechanism. In compared to standard sample, more stable Coefficientoffriction was achieved for laser-optimized sample. After evaluating graphene oxide coatings, it could be concluded that suspension with 0.5 g/li isopropanol and optimized electrophoretic factors (90 V and 10 min) brought about to form more uniform coating rather than other coating parameters. Moreover, uniform coating was reached as polyvinylpyrrolidones (0.5 gr/li polyvinylpyrrolidones and 0.3 gr/li tanic acid in ethanol, 30 V and 10 min) was added into the suspension. In following, two layer nanocomposite coating was prepared by using two stage electrophoretic process (graphene oxide and then polyvinylpyrrolidones). The results obtained from 5 days of cell culture of Mg63 cells showed that two layer nanocomposite coating had higher cell viability (185±27 %) than that of the sample subjected to laser and graphene oxide coating. Furthermore, the result of friction test has illustrated that an increase in applying load prompted to increase coefficient of friction in control sample. However, it caused to decrease coefficient of friction in optimal sample prepared by laser and graphene oxide deposition. Additionally, in optimal load of 4 N, in compared to three times reduction in control sample, significant reduction (about 11 times) in coefficient of friction of optimal sample was observed. Generally, it could be inferred that applying both surface modification and lubricant coating simultaneously accompanied by tribological and biological improvements might be useful in applications of knee joints implants. Key words: knee joint implant, Ti6Al4V alloy, tribology, surface modification, electrophoretic