Today, nanotechnology plays a vital role in medical applications, especially for diagnosis and treatment of diseases. One of the medical topics is genetherapy that involves the prevention or treatment of serious diseases such as inherited disorders and many types of cancers and viral infections that genetic material is used for. Among different types of nanoparticles, magnetite nanoparticles (Fe 3 O 4 ), that have high magnetization compared to other particles are considered as unique particles for medical applications including imaging and treatment (genetherapy and drugdelivery). Magnetice nanoparticles coated with polymer have been made in recent decades and are so interesting subject for researchers. In this study, magnetite iron oxide nanoparticles containing pcDNA3.1(+) plasmid and coated with Quince seed polymer, which is a natural and non-toxic coating and also a Calcium chloride deposition agent were synthesized. Then its application in genedelivery as a magnetic gene carrier was investigated. Coated nanoparticles were characterized by several tools such as X-ray diffraction, Zeta potential analysis to check stability, dispersion and determination of electrostatic charge, analyzing and measuring the hydrodynamic diameter of nanoparticles, analyzing infrared spectroscopy, field-scanning electron microscopy, magnetization vibration test and electrophoresis (method used for genetherapy) to investigate their structural and magnetic properties. The results showed that the best way to form the suspend nanoparticles is to tune the calcium chloride at pH=7 and concentration of 0.1%. The analysis of field-scanning electron microscopy of nanoparticles showed a spherical structure for nanoparticles with size of 50nm. They showed a Maximum absorption of the pcDNA3.1(+) plasmid and a good stability in an environment with acid pH (similar to the gastric environment) they could release up to 99% in an environment with an alkaline pH (similar to the intestine environment) and their transfer to target tissue or cell is possible.
