In recent years a new approach to skin care is emerging-cosmetotextiles. Cosmetotextiles combine textile materials with cosmetic active substance. Microcapsulation, not only protects cosmetics materials against various factors (such as heat, oxidation, evaporation and degradation) but also, it improves their ability to penetrate into the skin. Silk fibroin and Bovine Serum Albumin are natural polymers with nutritional and drug properties that has been studied for numerous applications.A bout 10% of the total produced silk in iran is sold as the waste material to the abroad at very low price.This saliency is created the predispose to use of this valuable material to produce materials in medical field, filter preparation and biological materials that can be grounds for significant added value. In addition, protein such as albumin because of properties for instance biodegradability, biocompatibility, non- toxicity and high stability in the body have been known as systems for drug delivery among other systems. In this research, protein microspheres of Bovine Serum Albumin (BSA) and silk fibroin contain Tocopherol acetate were produced via ultrasonic emulsification and then applied on cotton fabric for cosmeto textiles applications. Nano particles with particle size of 200-300 nm with entrapment efficiencies of around 99% were obtaind. In recent years a new approach to skin care is emerging-cosmetotextiles. Cosmetotextiles combine textile materials with cosmetic active substance. Microcapsulation, not only protects cosmetics materials against various factors (such as heat, oxidation, evaporation and degradation) but also, it improves their ability to penetrate into the skin. Silk fibroin and Bovine Serum Albumin are natural polymers with nutritional and drug properties that has been studied for numerous applications.A bout 10% of the total produced silk in iran is sold as the waste material to the abroad at very low price.This saliency is created the predispose to use of this valuable material to produce materials in medical field, filter preparation and biological materials that can be grounds for significant added value. In addition, protein such as albumin because of properties for instance biodegradability, biocompatibility, non- toxicity and high stability in the body have been known as systems for drug delivery among other systems. In this research, protein microspheres of Bovine Serum Albumin (BSA) and silk fibroin contain Tocopherol acetate were produced via ultrasonic emulsification and then applied on cotton fabric for cosmeto textiles applications. Nano particles with particle size of 200-300 nm with entrapment efficiencies of around 99% were obtaind. Results showed that nanoparticles had good antioxidant activity with no significant cytotoxicity to human skin keratinocytes for all the concentrations tested. In the next step, cotton fabrics were coated with protein- based nanoparticles containing vitamin E (?-Tocopherol) by pad – cure method. SEM, FTIR and physical adsorption tests, fastness (to washing, rubbing, protease and perspiration) and air permeability analysis of coated samples confirmed the fixation of the nanoparticles on to the fabric’ s surface. The protein- based coating was maintained for at least 10 washing cycles demonstrating the reliability of the pad- cure method for the fixation of nanoparticles on to cotton surfaces. A methodology for nanoparticles release from the coated surfaces and their transfer to other substrates, was demonstrates by the simple Crockmeter rubbing in the presence of sweat and protease. A high amount of material can be transferred and released to other substrates like textiles and skin by a synergistic effect of sweat/ protease and abrasion. Overall, the results showed that BSA:VO and BSA:SF:VO nanoparticles can be used as biodegradable and non-toxic nanoformulations for the functionalization of textile and control delivery of Tocopherol into the skin.The surface modification of cellulose was also done to improve the nanoparticles loading efficiency of fabrics Keywords : cosmetotextiles, Silk fibroin (SF), Bovine Serum Albumin (BSA), Tocopherol acetate, antioxidant activity.