This study investigated Holstein dairy calves response to enriched colostrum and milk with hormone and mineral-vitamin (min-vit) supplemented growth factors. Forty Holstein (first BW 40.9 ± 2) dairy calves were arranged to four treatments from birth to weaning based on sex and randomly including; 1) control, 2) hormone composition, 3) mineral-vitamin supplement and 4) a mix of 2 and 3 treatments. The hormone composition consisted of growth hormone, insulin-like growth factor I, insulin-like growth factor II, prolactin, glucagon and insulin (2.5-3 times more than normal colostrum concentration). Colostrums enrichment with vitamin-mineral supplement consisted of selenium-methionine; zinc-methionine and fat soluble vitamins A, E, D (double NRC recommendation). Calves in each group were fed treatments soluble in colostrum or milk for 5 day after birth and after that calves had adlibitum access to a starter concentrate and the starter consumption was measured every day for every individual calf. Before weaning calves received 4 liter whole milk daily. Blood samples were taken after birth and before feeding colostrum on d 1 and 30 min after feeding colostrum or milk on d 2, 3 and 20. One calf of each group (have average performance) were slaughtered on 60 day of life for morphometrical analyses. The rumen, abomasum, small intestine were dissected for sampling. Hormone or min-vit supplement showed weaning age reduction (p 0.05) for 6 days and a large amount starter daily intake increment (p 0.0001) but there were no significant differences between groups in the weaning weight, weaning Withers height, average daily gain. Difference between globulin%, albumin% and the albumin to globulin ratio was not significant in blood serum analyses. It also revealed that colostrums enrichment over the early ages had a marked effect on rumen villus, papillae and apparent maturity of this tissue. Morphometric analyses showed colostrums enrichment enhanced small intestinal growth and development in neonatal calves. There were no differences in protein concentrations between treatment groups in the segments of rumen (ventral and dorsal) and abomasums. In conclusion, the hormone composition alone or with mineral-vitamin supplement has better effects on performance and morphological traits than colestrum and milk without any enrichment or with mineral-vitamin supplement alone in neonatal calves. This study confirms that enriched colostrum with hormone or min-vit supplement may improve calves performance with decreasing the weaning age, starter daily intake and enhancing growth and development gastrointestinal tract and the calves may become a ruminant sooner than expected but these supplement cannot influence on albumin to globulin ratio and immunity system. Key words: hormone, mineral-vitamin supplement, performance,